Insulin Protects Pancreatic Acinar Cells from Palmitoleic Acid-induced Cellular Injury

Insulin protects pancreatic acinar cells

Background: Impaired metabolism and cytosolic Ca overload in pancreatic acinar cells can trigger pancreatitis. Results: Insulin protected cells from oxidantinduced Ca overload, inhibition of the plasma membrane calcium pump (PMCA) and ATP depletion. Conclusion: Insulin switches metabolism towards glycolysis and fuels the PMCA even when mitochondria are impaired. Significance: This mechanism may...

Phenylpropenoic Acid Glucoside from Rooibos Protects Pancreatic Beta Cells against Cell Death Induced by Acute Injury

OBJECTIVE Previous studies demonstrated that a phenylpropenoic acid glucoside (PPAG) from rooibos (Aspalathus linearis) extract had anti-hyperglycemic activity and significant protective effects on the pancreatic beta cell mass in a chronic diet-induced diabetes model. The present study evaluated the cytoprotective effect of the phytochemical on beta cells exposed to acute cell stress. METHOD...

Bach1 deficiency protects pancreatic β-cells from oxidative stress injury.

BTB and CNC homology 1 (Bach1) is a transcriptional repressor of antioxidative enzymes, such as heme oxygenase-1 (HO-1). Oxidative stress is reportedly involved in insulin secretion impairment and obesity-associated insulin resistance. However, the role of Bach1 in the development of diabetes is unclear. HO-1 expression in the liver, white adipose tissue, and pancreatic islets was markedly upre...

Pancreatic Acinar Cells

Lineage tracing and characterization of insulin-secreting cells generated from adult pancreatic acinar cells.

Although several studies have suggested that insulin-secreting cells can be generated in vitro from cells residing in adult exocrine pancreas, neither the origin of these cells nor their precise insulin secretory properties was obtained. We show here that insulin-secreting cells can be derived from adult mouse pancreatic exocrine cells by suspension culture in the presence of EGF and nicotinami...